Improved BASIL Workflow Provides Precise and Robust Protein Profiling for Single-Cell Analysis

Improved BASIL Workflow Provides Precise and Robust Protein Profiling for Single-Cell Analysis     Almost 18 months ago, CPTAC researchers from the Integrative Omics Group at Pacific Northwest National Laboratory (PNNL), developed a stream-lined technique to increase the sensitivity of peptide phospho-group identification during mass spectral analysis called the 'boosting to amplify signal with isobaric labeling' (BASIL). Well, they're back again with an improved BASIL (iBASIL) strategy that allows for the same increased peptide sensitivity – but on a single-cell level! The iBASIL methodology demonstrates a way to look at single cells with the coverage and accuracy that rivals typical bulk analysis methods, paving the way for research solutions to important biological questions like tumor heterogeneity. Read more about it here! The Office of Cancer Clinical Proteomics Research (OCCPR) aims to improve prevention, early detection, diagnosis, and treatment of cancer by enhancing the understanding of the molecular mechanisms of cancer, advancing proteome/proteogenome science and technology development through community resources (data and reagents), and accelerating the translation of molecular findings into the clinic. Sign-up for email updates about the CPTAC program here.

 

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